Table of Contents

Growth Protocols for Model Plants in Developmental Biology.- Grafting as a Research Tool.- Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function.- The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level.- Inducible Gene Expression Systems for Plants.- Trichome Development in Arabidopsis.- Phenotyping the Development of Leaf Area in Arabidopsis thaliana.- Analyzing Shoot Apical Meristem Development.- Analyzing Floral Meristem Development.- Female Gametophytic Mutants: Diagnosis and Characterization.- Pollen Tube Development.- Analysis of Root Meristem Size Development.- Phenotypic Characterization of Photomorphogenic Responses During Plant Development.- Kinematic Analysis of Cell Division and Expansion.- Flowering Time Control.- mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis.- Immunolocalization of Proteins in Plants.- Detection of Small Non-coding RNAs.- Quantitative Real Time PCR in Plant Developmental Biology.- Luciferase and Green Fluorescent Protein Reporter Genes as Tools to Determine Protein Abundance and Intracellular Dynamics.- Fluorescence-Activated Cell Sorting in Plant Developmental Biology.- Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling.- Utilizing Bimolecular Fluorescence Complementation (BiFC) to Assay Protein–Protein Interaction in Plants.- The Split Luciferase Complementation Assay.- Co-immunoprecipitation and Protein Blots.- Probing Protein–Protein Interactions with FRET–FLIM.- Plant Chromatin Immunoprecipitation.- Immunocytological Analysis of Chromatin in Isolated Nuclei.- Bisulphite Sequencing of Plant Genomic DNA.