Table of Contents

1.Collection and handling of blood

2. Reference ranges and normal values

3. Basic haematological techniques

4. Preparation and staining methods for

blood and bone marrow films

5. Blood cell morphology in health and

disease

6. Supplementary techniques including

blood parasite diagnosis

7. Bone marrow biopsy

8. Molecular and cytogenetic analysis

9. Iron deficiency anaemia and iron

overload

10. Investigation of megaloblastic anaemia:

cobalamin, folate and metabolite

status

11. Laboratory methods used in the investigation

of the haemolytic anaemias

12. Investigation of the hereditary haemolytic

anaemias: membrane and enzyme

abnormalities

13. Acquired haemolytic anaemias

14. Investigation of abnormal haemoglobins

and thalassaemia

15. Erythrocyte and leucocyte

cytochemistry

16. Immunophenotyping

17. Diagnostic radioisotopes in

haematology

18. Investigation of haemostasis

19. Investigation of a thrombotic

tendency

20. Laboratory control of anticoagulant,

thrombolytic and antiplatelet

therapy

21. Blood cell antigens and antibodies:

erythrocytes, platelets and

granulocytes

22. Laboratory aspects of blood

transfusion

23. Approach to the diagnosis and

classification of blood diseases

24. Laboratory organization and

management

25. Quality assurance

26. Haematology in under-resourced

laboratories

27. Appendix