Table of Contents

Benson's Microbiological Applications, Laboratory Manual in General Microbiology, Complete Version, 10th Edition Part 1 Microscopy Exercise 1 Brightfield Microscopy Exercise 2 Darkfield Microscopy Exercise 3 Phase-Contrast Microscopy Exercise 4 Fluorescence Microscopy Exercise 5 Microscopic Measurements Part 2 Survey of Microorganisms Exercise 6 Protozoans, Algae, and Cyanobacteria Exercise 7 Ubiquity of Bacteria Exercise 8 The Fungi: Yeasts and Molds Part 3 Manipulation of Microorganisms Exercise 9 Aseptic Technique Exercise 10 Pure Culture Techniques Part 4 Staining and Observation of Microorganisms Exercise 11 Smear Preparation Exercise 12 Simple Staining Exercise 13 Negative Staining Exercise 14 Capsular Staining Exercise 15 Gram Staining Exercise 16 Spore Staining: Two Methods Exercise 17 Acid-Fast Staining: Ziehl-Neelsen Method Exercise 18 Motility Determination Part 5 Culture Methods Exercise 19 Culture Media Preparation Exercise 20 Preparation of Stock Cultures Exercise 21 Cultivation of Anaerobes Exercise 22 Enumeration of Bacteria: The Standard Plate Count Exercise 23 Slime Mold Culture Exercise 24 Slide Culture: Molds Part 6 Bacterial Viruses Exercise 25 Determination of a Bacteriophage Titer Exercise 26 Burst Size Determination: A One-Step Growth Curve Exercise 27 Isolation of Phage from Flies Exercise 28 Phage Typing Part 7 Enviornmental Influences and Control of Microbial Growth Exercise 29 Temperature: Effects on Growth Exercise 30 Temperature: Lethal Effects Exercise 31 pH and Microbial Growth Exercise 32 Water Activity and Osmotic Pressure Exercise 33 Ultraviolet Light: Lethal Effects Exercise 34 Oligodynamic Action Exercise 35 Evaluation of Alcohol: Its Effectiveness As an Antiseptic Exercise 36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method Exercise 37 Evaluation of Antiseptics: The Filter Paper Disk Method Exercise 38 Effectiveness of Hand Scrubbing Part 8 Identification of Unknown Bacteria Exercise 39 Morphological Study of Unknown Bacterium Exercise 40 Cultural Characteristics Exercise 41 Physiological Characteristics: Oxidation and Fermentation Tests Exercise 42 Physiological Characteristics: Hydrolytic Reactions Exercise 43 Physiological Characteristics: Biochemical Tests Exercise 44 Use of Bergey's Manual Part 9 Miniaturized Multitest Systems Exercise 45 Enterobacteriaceae Identification: The API 20E System Exercise 46 Enterobacteriaceae Identification: The Enterotube II System Exercise 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System Exercise 48 Staphyococcus Identification: The API Staph-Ident System Part 10 Diversity and Environmental Microbiology Exercise 49 Isolation of an Antibiotic Producer: The Actinomyces Exercise 50 Nitrogen Cycle: Ammonification Exercise 51 Symbiotic Nitrogen Fixation: Rhizobium Ex

About the Author

Professor and Chair, Consultant Environmental Microbiology