Table of Contents

01. Introduction 02. Principles of Cell Culture 03. The Cell and its Environment 04. Types of Cells And Cell Culture 05. Cell Culture: Applications, Advantages and Disadvantages 06. Ideal Cell Culture Laboratory with Equipments 07. Growth and Maintenance of Cells in Culture 08. Components of Medium and its Preparation 09. Primary Cell Culture 10. Maintenance of Cell Culture 11. Packaging and Transportation of Cells 12. Description of Commonly Used Cell Lines 13. Subculturing of Cells in Different Vessels 14. Scaling Up of Animal Cell Culture 15. Cell Line Authentication and Characterization 16. Cryopreservation and Thawing/Revival of Cells 17. Contamination of Cell Culture and Curing 18. Counting of Cells 19. Isolation and Identification of Virus in Cell Culture 20. Virus Assays 21. Collection, Preservation and Transport of Various 22. Specimens for Laboratory Diagnosis 23. Collection of Materials for Diagnosis of Viral 24. Diseases 25. Processing of Laboratory Specimens, Inoculation into Laboratory Animals and Embryonated Eggs for Pathogen Isolation 26. Serum Neutralization Test 27. Enzyme Linked munosorbent Assay ELISA 28. Biosafety Measures and Risk Management