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Understanding PCR


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A user friendly book that features step-by-step basic protocols, helpful resource recommendations, and basic troubleshooting tips, this approachable and practical guide explains the process for setting up and analyzing one's first PCR reaction

Table of Contents

Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers Chapter 3. Analyzing Your Template DNA and/or PCR Product Chapter 4. Quantitative PCR: Things to Consider Chapter 5. Carrying Out Q-PCR Chapter 6. Using PCR for Cloning and Protein Expression Chapter 7. Polymerase Chain Reaction for Knocking Out Genes

About the Author

Sarah Maddocks, PhD, is a lecturer in Microbiology at Cardiff Metropolitan University, UK. Her research focuses on understanding the interactions between pathogens and their host during the infection process. The overarching aim of these studies is to understand how pathogens utilise or disrupt host processes and to explore and exploit novel ways of impairing these host-pathogen interactions to facilitate clearance of infection. Dr. Maddocks' research also includes biofilm and co-culture models, genomic and molecular analysis of gene expression to prove clinically relevant hypotheses. Rowena Jenkins, PhD, is a lecturer in Microbiology and Infectious Disease in the College of Medicine at Swansea University, UK. Her research team are focused on the effect of novel antimicrobial agents on pathogenic bacteria found in clinical infections, particularly chronic infections such as those seen in diabetic foot ulcers and cystic fibrosis lungs infection, and the impact on the microbiome within those conditions. Dr. Jenkins also has an interest in the potential for natural antimicrobial agents to improve the efficacy of conventional antibiotics against antibiotic resistant bacteria and biofilms.

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