How does Fishpond Work?

We won't be beaten by anyone. Guaranteed

Techniques in Prion Research
By

Rating

Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), exist in both humans (Creutzfeldt-Jakob disease (CJD)) and animals (scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease) and have the unique property of being infectious, sporadic or genetic in origin.

Although the precise nature of the infectious agent responsible for TSEs is not definitely identified, it is now clearly demonstrated that a protein named PrP (for Prion Protein) plays a critical role in the transmission and pathogenesis of TSEs.

This book provides the general description as well as the details of the techniques currently used for the study of prion diseases. Taking into account the pivotal role played by PrP it is not surprising that many Chapters of this book deal with the purification, the detection and the characterization of the different forms of this protein. In addition, in vitro, cellular and animal models specifically adapted to the study of TSEs, as well as bio-safety procedures are described. Each Chapter is written by scientists involved for many years in their respective domain of prion biology who give the best of their knowledge in this technical document. This volume is a very useful tool for any laboratory which recently decided to contribute to the study of TSEs as well as for teams already engaged in this field for many years but interested in extending their technical capacity toward new methods.

Features:

Purification of PrPC and the Pathological Isoform of Prion Protein (PrPsc or PrP-res)

Animal Models of TSEs

Cell Culture Models of TSEs

PrPsc Immunohistochemistry

Western Immunoblotting Techniques

Antibody Production and ELISA

TSE Strain Typing in Mice

Biosafety and Decontamination Procedures

Cell-free Conversion of Prion Proteins

Cytotoxicity of PrP Peptides

Cyclic Amplification of Prion Protein Misfolding

Of interest to:

Researchers and clinicians in the fields of cell biology, biomedicine, neuroscience/neuropathology, veterinary medicine and biochemistry.

Product Details

Table of Contents

From the contents: Abbreviations.- Transmissible Spongiform Encephalopathies or Prion Diseases (General Introduction).- Purification of PrPC.- Purification of the Pathological Isoform of Prion Protein (PrPsc or PrP-res) from Transmissible Spongiform Encephalopathy-affected Brain Tissue. Rapid Procedure for Purification and Renaturation of Recombinant PrP Protein.- Animal Models of Transmissible Spongiform Encephalopathies: Experimental Infection, Observation and Tissue Collection.- Cell Culture Models of TSEs. PrPsc Immunohistochemistry.- Western Immunoblotting Techniques for the Study of Transmissible Spongiform Encephalopathies.- Antibody Production and ELISA, Diagnosis of TSEs, General Considerations.- TSE Strain Typing in Mice.- Biosafety and Decontamination Procedures.- Cell-free Conversion of Prion Proteins.- Cytotoxicity of PrP Peptides.- Cyclic Amplification of Prion Protein Misfolding. Guide to Solutions.- Guide to Protocols.- Index

Promotional Information

Springer Book Archives

Reviews

This is an excellent book for the description of specific methods for use in TSE research. The reason why such a book is necessary is because around the world people have been using different methods frequently and it has become extremely difficult to repeat other researcher's methods. One of the reason has also been a determination by researchers not to supply samples or data to other researchers. However this book is like a breath of fresh air in all that with an extremely good description of how it should be done with everything from transmission systems to cell-free conversion of prion proteins laid out as complete systems. Remarkably some of the methods I would have expected to have been almost 'private' in that the researchers did not want other people to know the method...but not here! Clearly the editors, Sylvain Lehmann and Jacques Grassi had not let the writers miss things out and full systems are mapped out. Full marks to the editors and the writers in this one and an excellent book that should be in the hands of everyone in the field. (http://www.priondata.org/data/A_book.htm)

Look for similar items by category
Home » Books » Science » Medicine » General
Home » Books » Science » Medicine » Immunology
Home » Books » Science » Medicine » Diseases
Home » Books » Science » Medicine » Neuroscience
Home » Books » Science » Medicine » Research
People also searched for
How Fishpond Works
Fishpond works with suppliers all over the world to bring you a huge selection of products, really great prices, and delivery included on over 25 million products that we sell. We do our best every day to make Fishpond an awesome place for customers to shop and get what they want — all at the best prices online.
Webmasters, Bloggers & Website Owners
You can earn a 5% commission by selling Techniques in Prion Research (Methods and Tools in Biosciences and Medicine) on your website. It's easy to get started - we will give you example code. After you're set-up, your website can earn you money while you work, play or even sleep! You should start right now!
Authors / Publishers
Are you the Author or Publisher of a book? Or the manufacturer of one of the millions of products that we sell. You can improve sales and grow your revenue by submitting additional information on this title. The better the information we have about a product, the more we will sell!
Item ships from and is sold by Fishpond World Ltd.
Back to top