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Seed Dormancy
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Understanding seed-related processes is of major social, environmental, and economic concern. The viability and vigor of seeds are the very basis for sustainable agriculture and forestry, and comprehending the molecular and cellular events underlying these processes will become increasingly important to many economical sectors and for species that provide the world's food supply. Seed Dormancy: Methods and Protocols covers analytical methods and approaches which have already lead to significant advances in the understanding of seed dormancy and germination. Chapters cover explanations of processes leading to the induction, maintenance, and termination of seed dormancy, the classification of different dormancy types, as well as an overview of protocols used for dormancy-termination of seeds of conifer species. This volume emphasizes methods essential for abscisic acid (ABA) analyses, including methods that have been important for receptor identification, analyses of ABA-catabolizing enzymes (the 8'-hydroxylases), and identification of novel signal transduction components, interacting partners, and/or response factors. The volume closes by addressing the development of new technologies, including spectroscopic methods (some of which allow for non-destructive sampling) as well as highly effective tissue-printing methods for seed dormancy research. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Seed Dormancy: Methods and Protocols features detailed methods that will prove invaluable for both applied and fundamental seed research.
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Table of Contents

I. Introduction 1. Challenges Facing the Forest Industry in Relation to Seed Dormancy and Seed Quality Michael U. Stoehr and Yousry A. El-Kassaby 2. Challenges Facing Seed Banks and Agriculture in Relation to Seed Quality M. Veronica Rodriguez, Peter E. Toorop, and Roberto L. Benech-Arnold II. Breaking Seed Dormancy and Standardizing Seed Dormancy Research 3. Standardizing Seed Dormancy Research Henk WM Hilhorst 4. Eyeing Emergence: Modified Treatments for Terminating Dormancy of Conifer Seeds J. Allan Feurtado and Allison R. Kermode 5. Production of Seed Samples for the Effective Molecular Analysis of Dormancy Cycling in Arabidopsis Steven Footitt and William E. Finch-Savage III. Hormone Analyses 6. Approaches to the Identification of ABAR as an Abscisic Acid Receptor Xiao-Fang Wang, Qi Xin, Yuan-Yue Shen, and Da-Peng Zhang 7. Profiling of Hormones and Related Metabolites in Seed Dormancy and Germination Studies Mitsunori Seo, Yusuke Jikumaru, and Yuji Kamiya 8. In Vitro Assay for ABA 8'-Hydroxylase: Implications for Improved Assays for Cytochrome P450 Enzymes Joan E. Krochko and Adrian J. Cutler 9. Functional Analysis of Abscisic Acid 8'-Hydroxylase Akira Endo, Mitsuhiro Kimura, Naoto Kawakami, and Eiji Nambara IV. Gene, RNA, and Protein Analyses 10. Isolation of Genetic Material from Arabidopsis Seeds Urszula Piskurewicz and Luis Lopez-Molina 11. Identification and Characterization of Quantitative Trait Loci (QTL) that Control Dormancy in Arabidopsis Leonie Bentsink and Maarten Koornneef 12. Identification of Seed Dormancy Mutants by Activation Tagging Tiehan Zhao, Ying Zeng, and Allison R. Kermode 13. Isolation of MicroRNAs that Regulate Seed Dormancy and Germination Arun Kumar M.B., Ruth C. Martin, and Hiro Nonogaki 14. Proteomics and Post-Translational Proteomics of Seed Dormancy and Germination Loic Rajjou, Maya Belghazi, Julie Catusse, Laurent Oge, Erwann Arc, Beatrice Godin, Kamel Chibani, Sonia Ali-Rachidi, Boris Collet, Philippe Grappin, Marc Jullien, Karine Gallardo, Claudette Job, and Dominique Job V. Epigenetic Changes Associated with Seed Dormancy 15. Chromatin Dynamics during Seed Dormancy Yongxiu Liu, Regina Geyer, Vittoria Brambilla, Kazumi Nakabayashi, and Wim J.J. Soppe 16. Emerging Mass Spectrometry-Based Technologies for Analyses of Chromatin Changes: Analysis of Histones and Histone Modifications Brinda Shah, Rachel L. Kozlowski, and Christoph H. Borchers VI. Spectroscopic and Whole Tissue Analyses 17. In Vivo Nuclear Magnetic Resonance (NMR) Metabolite Profiling in Plant Seeds Victor Terskikh and Allison R. Kermode 18. In Vivo 1H NMR Microimaging during Seed Imbibition, Germination, and Early Growth Victor Terskikh, Kerstin Muller, Allison Kermode, and Gerhard Leubner-Metzger 19. Tissue Printing Methods for Localization of RNA and Proteins that Control Seed Dormancy and Germination Wioletta E. Pluskota, Kent J. Bradford, and Hiro Nonogaki VII. Analyses of Reactive Oxygen Species, Protein Damage and Repair 20. Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination Christophe Bailly and Ilse Kranner 21. Protein Damage and Repair Controlling Seed Vigor and Longevity Laurent Oge, Caroline Broyart, Boris Collet, Beatrice Godin, Denis Jallet, Gildas Bourdais, Dominique Job, and Philippe Grappin 22. Nitric Oxide Methods in Seed Biology Paul C. Bethke, Igor Libourel, Jan Vitecek, and Russell L. Jones VIII. Bioinformatic Resources 23. Seed Bioinformatics George W. Bassel, Michael J. Holdsworth, and Nicholas J. Provart

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