Fishpond Gift Vouchers - Let them choose!

Shop over a million Toys in our Huge New Range

Retinoids
By

Rating
New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A's impact on the human body.
Product Details

Table of Contents

1. Quantification of Endogenous Retinoids Maureen A. Kane and Joseph L. Napoli 2. Culture of Highly Differentiated Human Retinal Pigment Epithelium for Analysis of the Polarized Uptake, Processing, and Secretion of Retinoids Jane Hu and Dean Bok 3. Feeder Independent Culture of Mouse Embryonic Stem Cells Using Vitamin A/Retinol Jaspal S. Khillan and Liguo Chen 4. In Vitro Assays of Rod and Cone Opsin Activity: Retinoid Analogues as Agonists and Inverse Agonists Masahiro Kono and Rosalie K. Crouch 5. Physiological Studies of the Interaction between Opsin and Chromophore in Rod and Cone Visual Pigments Vladimir J. Kefalov, M. Carter Cornwall, and Gordon L. Fain 6. Measurement of the Mobility of All-Trans Retinol with Two-Photon Fluorescence Recovery after Photobleaching Yiannis Koutalos 7. Microfluorometric Measurement of the Formation of All-Trans Retinol in the Outer Segments of Single Isolated Vertebrate Photoreceptors Yiannis Koutalos and M. Carter Cornwall 8. HPLC/MSN Analysis of Retinoids James E. Evans and Peter McCaffery 9. Binding of Retinoids to ABCA4, the Photoreceptor ABC Transporter Associated with Stargardt Macular Degeneration Ming Zhong and Robert S. Molday 10. Fluorescence-Based Technique for Analyzing Retinoic Acid Leslie J. Donato and Noa Noy 11. The Interaction Between Retinol-Binding Protein and Transthyretin Analyzed by Fluorescence Anisotropy Claudia Folli, Roberto Favilla, and Rodolfo Berni 12. Assay of Retinol Binding Protein-Transthyretin Interaction and Techniques to Identify Competing Ligands Nathan L. Mata, Kim Phan, and Yun Han 13. Molecular Biology and Analytical Chemistry Methods Used to Probe the Retinoid Cycle Marcin Golczak, Grzegorz Bereta, Akiko Maeda, and Krzysztof Palczewski 14. Visualization of Retinoid Storage and Trafficking by Two-Photon Microscopy Yoshikazu Imanishi and Krzysztof Palczewski 15. Reverse-Phase High Performance Liquid Chromatography (HPLC) Analysis of Retinol and Retinyl Esters in Mouse Serum and Tissues Youn-Kyung Kim and Loredana Quadro 16. Detection of Retinoic Acid Catabolism with Reporter Systems and by In-Situ Hybridization for CYP26 Enzymes Yasuo Sakai and Ursula C. Drager 17. Diet in Vitamin A Research A. Catharine Ross 18. Experimental Approaches to the Study of A2E, a Bisretinoid Lipofuscin Chromophore of Retinal Pigment Epithelium Janet R. Sparrow, So Ra Kim, and Yalin Wu 19. Analysis of the Retinoid Isomerase Activities in the Retinal Pigment Epithelium and Retina Gabriel H. Travis, Joanna Kaylor, and Quan Yuan 20. Techniques to Study Specific Cell-Surface Receptor-Mediated Cellular Vitamin A Uptake Riki Kawaguchi and Hui Sun

Reviews

From the reviews: "This excellent book provides authoritative and cutting-edge information in the field of retinoids ... . It is intended both for beginning and experienced researchers in diverse fields who are interested in learning new techniques. Even graduate students, nutritionists, and clinicians could find this book informative and helpful. ... By combining biochemical, biophysical, and cell biological techniques, the book thoroughly addresses the field of retinoid research." (Omer Iqbal, Doody's Review Service, October, 2010)

Look for similar items by category
Home » Books » Science » Medicine » Oncology
Home » Books » Science » Biology » Molecular
Home » Books » Science » Biology » Biochemistry
Home » Books » Science » Biology » Biophysics
How Fishpond Works
Fishpond works with suppliers all over the world to bring you a huge selection of products, really great prices, and delivery included on over 25 million products that we sell. We do our best every day to make Fishpond an awesome place for customers to shop and get what they want — all at the best prices online.
Webmasters, Bloggers & Website Owners
You can earn a 5% commission by selling Retinoids: Methods and Protocols (Methods in Molecular Biology) on your website. It's easy to get started - we will give you example code. After you're set-up, your website can earn you money while you work, play or even sleep! You should start right now!
Authors / Publishers
Are you the Author or Publisher of a book? Or the manufacturer of one of the millions of products that we sell. You can improve sales and grow your revenue by submitting additional information on this title. The better the information we have about a product, the more we will sell!
Item ships from and is sold by Fishpond World Ltd.
Back to top