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Practical Guide to Diagnostic Parasitology


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Table of Contents Section 1. Philosophy and Approach to Diagnostic Parasitology Why Perform This Type of Testing? Who Should Perform Diagnostic Parasitology Testing? Where Should Diagnostic Parasitology Testing Be Performed? What Factors Should Precipitate Testing? What Testing Should Be Performed? What Factors Should Be Considered When Developing Test Menus? Risk Management Issues Associated with STAT Testing Section 2. Parasite Classification and Relevant Body Sites Protozoa (Intestinal) Protozoa (Other Body Sites) Protozoa (Blood and Tissue) Nematodes (Intestinal) Nematodes (Tissue) Nematodes (Blood and Tissue) Cestodes (Intestinal) Cestodes (Tissue) Trematodes (Intestinal) Trematodes (Liver and Lungs) Trematodes (Blood) Pentastomids Acanthocephala Table 2.1 Classification of Human Parasites Table 2.2 Cosmopolitan Distribution of Common Parasitic Infections Table 2.3 Body Sites and Possible Parasites Recovered Section 3. Collection Options Safety Collection of Fresh Stool Specimens Preservation of Stool Specimens Collection of Blood Collection of Specimens from Other Body Sites Table 3.1 Fecal Specimens for Parasites: Options for Collection and Processing Table 3.2 Approaches to Stool Parasitology: Test Ordering Table 3.3 Preservatives and Procedures Commonly used in Diagnostic Parasitology (Stool Specimens) Table 3.4 Advantages of the Thin and Thick Blood Films Table 3.5 Advantages and Disadvantages of Buffy Coat Films Table 3.6 Potential Problems of Using EDTA Anticoagulant for the Preparation of Thin and Thick Blood Films Table 3.7 Body Sites and Possible Parasites Recovered Section 4. Specimen Test Options: Routine Diagnostic Methods and Body Sites Ova and Parasite Examination of Stool Specimens Other Diagnostic Methods for Stool Specimens Testing of Other Intestinal Tract Specimens Urogenital Tract Specimens Sputum Aspirates Biopsy Specimens Blood Culture Methods Animal Inoculation and Xenodiagnosis Antibody and Antigen Detection Table 4.1: Body Site, Procedures and Specimens, Recommended Methods and Relevant Parasites, and Comments Table 4.2: Serologic, Antigen, and Probe Tests Used in the Diagnosis of Parasitic Infections Section 5. Specific Test Procedures and Algorithms Microscopy Ova and Parasite Examination Fecal Immunoassays for Intestinal Protozoa Larval Nematode Culture Other Methods for Gastrointestinal Tract Specimens Methods for Urogenital Tract Specimens Preparation of Blood Films Blood Stains Blood Concentration Algorithm 5.1: Procedure for Processing Fresh Stool for the O&P Examination Algorithm 5.2: Procedure for Processing Liquid Specimens for the O&P Examination Algorithm 5.3: Procedure for Processing Preserved Stool for the O&P Examination-Two-Vial Collection Kit Algorithm 5.4: Procedure for Processing SAF-Preserved Stool for the O&P Examination Algorithm 5.5: Use of Various Fixatives and Their Recommended Stains Algorithm 5.6: Ordering Algorithm for the Laboratory Examination for Intestinal Parasites Algorithm 5.7: Procedure for Processing Blood Specimens for Examination Table 5.1: Body Site, Specimen, and Recommended Stain(s) Table 5.2: Approaches to Stool Parasitology: Test Ordering Table 5.3: Laboratory Test Reports: Optional Comments Table 5.4: Parasitemia Determined from Conventional Light Microscopy: Clinical Correlation Section 6. Commonly Asked Questions about Diagnostic Parasitology Stool Parasitology Tissues or Fluids Blood Section 7. Parasite Identification Protozoa Nematodes Cestodes Trematodes Section 8. Identification Aids Tables 8.1 to 8.37 Identification Keys 8.1 to 8.4 Figures 8.1 to 8.3 Plates 8.1 to 8.4

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