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Plant Tissue Culture


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Provides laboratory exercises in plant tissue culture that demonstrate major educational concepts

Table of Contents

1. History of Plant Cell Culture 2. Setup of a Tissue Culture Laboratory 3. Media Components and Preparation 4. Explant Preparation 5. Contamination 6. Callus Induction 7. Regeneration and Morphogenesis 8. Woody Shrubs and Trees 9. Haploid Plants from Anther Culture 10. Embryo Rescue 11. Meristem Culture for Virus-Free Plants 12. In Vitro Propagation for Commercial Production of Ornamentals 13. Protoplast Isolation and Fusion 14. Agrobacterium-Mediated Transformation of Plants Appendix I: Useful Measurements II: Review of Solution Preparation III: List of Suppliers IV: Common Plant Tissue Culture Terms


"The strength of this book lies in its descriptions of essential concepts, introduction to a wide range of major applications, and simple protocols to provide empirical demonstrations. ...this book would be a good teaching resource reference for instructors wishing to design their own laboratory demonstations." --Phil Bregitzer, Germplasm Research Facility, in CROP SCIENCE SOCIETY OF AMERICA (June 2001) "...presents a structured programme using plant material that is available all year round. ...I was impressed with the gradual introduction of complex techniques and ideas. ...the book is well written...a useful contribution to teaching in plant tissue culture." -Ronald A. de Fossard in TRENDS IN PLANT SCIENCE (February 2001) Praise for the First Edition "This is an excellent laboratory manual. The student can learn many plant tissue culture techniques commonly used today." --HORTSCIENCE (1995) "This book is an excellent overview of important tissue culture techniques. It is highly recommended for laboratory courses in tissue culture and would be useful in research laboratories where tissue culture techniques are required. Practical aspects of tissue culture are explained in a clear and concise way. This is essential for the novice and useful for experienced individuals planning a new course or laboratory. The manual is commended for providing information required for successful tissue culture at the fingertips of students." --JOURNAL OF NATURAL RESOUCES AND LIFE SCIENCES EDUCATION (1993)

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