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Photochemical Probes in Biochemistry

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Table of Contents

1. Photolabeling Reagent Design.- 2. Nucleophilic Aromatic Photo Substitutions on Nitrophenyl Ethers. A New Photoaffinity Labelling Technique.- 3. Photochemistry of Aryl and Aroyl Azides: Application to Photo-labeling of Biological Systems.- 4. Membrane Protein Labelling with Photoreactive Phospholipid Analogues.- 5. 125J-ASA-PE, A Photoactivable, Radioactive Phospholipid-Analogue, Designed for Photolabeling Proteins in Contact with Phospholipid Head Groups. Selective Photolabeling of Subunits ? in Thermophilic ATPsynthase TF0.F1.- 6. Photoaffinity Labelling of Receptors of Melanocyte-Stimulating Hormone.- 7. Topographical Analysis of the Torpedo Marmorata Acetylcholine Receptor by Energy Transfer Photoaffinity Labeling Using Aryldiazonium Derivatives.- 8. Photosuicide Labelling.- 9. Antibiotic Photoaffinity Labeling Probes of Escherichiacoli Ribosomal Structure and Function.- 10. Dissection of the Ribosomal Peptidyl Transferase Center by Photoaffinity Labeling: P- and A-sites are Located at Different Positions in Domain V of 23 S RNA:.- 11. Studying the Cytoskeleton by Label Transfer Crosslinking: Uses and Limitations.- 12. RecA-Directed Hybridization of Psoralen-Monoadducted DNA Oligonucleotides to Duplex Targets.- 13. The Use of Psoralen-Photocrosslinking for the Analysis of the Chromatin Structure during Transcription.- 14. Metal Complexes as Photochemical Probes of DNA Structure.- 15. Laser-Induced Strand Break Formation of Polyuridylic Acid in the Presence and Absence of Tris (2,2?-Bipyridyl) Ruthenium Chloride and K2S2O8.- 16. Photonucleases.- 17. Sequence-Targeted Photochemical Reactions in Single-Stranded and Double-Stranded Nucleic Acids by Oligonucleotide-Photosensitizer Conjugates.- 18. Photofootprinting Analysis of Protein-DNA Interactions.- 19. Studies of the Binding and Biological Actions of Ethidium.- 20. Quantitative Photoaffinity Labeling of Escherichiacoli RNA Polymerase Transcription Complexes by Nascent RNA.- 21. "Caged" Compounds to Probe the Dynamics of Cellular Processes: Synthesis and Properties of some Novel Photosensitive P-2-Nitrobenzyl Esters of Nucleotides.- General Comments on the Use of Photochemical Probes in Biochemistry. Advantages, Problems and Pitfalls.- List of Participants.- Author Index.- Keyword Index.

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