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Lysosomes and Lysosomal Diseases
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This new volume of Methods in Cell Biology looks at methods for lysosomes and lysosomal diseases. Chapters focus upon practical experimental protocols to guide researchers through the analysis of multiple aspects of lysosome biology and function. In addition, it details protocols relevant to clinical monitoring of patients with lysosomal diseases. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.
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This new volume of Methods in Cell Biology looks at methods for analyzing lysosomes and lysosomal diseases

Table of Contents

1. Methods for Monitoring Lysosomal Morphology Bethan S. Kilpatrick, Emily R. Eden, Leanne N. Hockey, Clare E. Futter and Sandip Patel 2. A Rapid Method for the Preparation of Ultra-pure, Functional Lysosomes Using Funcionalised Superparamagnetic Iron Oxide Nanoparticles Mathew William Walker and Emyr Lloyd-Evans 3. TFEB and the CLEAR Network Carmine Settembre and Diego L. Medina 4. Biosynthesis, Targeting and Processing of Lysosomal Proteins: Pulse-Chase Labeling and Immune Precipitation Sandra Pohl and Andrej Hasilik 5. Measuring Lysosomal pH by Fluorescence Microscopy Johnathan Canton and Sergio Grinstein 6. Lysosome Fusion in Cultured Mammalian Cells Nicholas A. Bright, Lena Wartosch and J. Paul Luzio 7. RNAi Screens of Lysosomal Trafficking Salil Garg, Michael B. Brenner 8. Approaches for Plasma Membrane Wounding and Assessment of Lysosome-mediated Repair Responses Corrotte, M, Castro-Gomes, T, Koushik, A.B. and Andrews, N.W 9. Imaging Approaches to Measuring Lysosomal Calcium Anthony J. Morgan, Lianne C. Davis, Antony Galione 10. Lysosome Electrophysiology Xi Zoe Zhong and Xian-Ping Dong 11. Reconstitution of Lysosomal Ion Channels into Artificial Membranes Elisa Venturi and Rebecca Sitsapesan 12. Fluorescence Methods for Analysis of Interactions Between Ca2+ Signalling, Lysosomes and Endoplasmic Reticulum David L. Prole, Cristina I. Lopez-Sanjurjo, Stephen C. Tovey and Colin W. Taylor 13. Methods for the Quantification of Lysosomal Membrane Permeabilization: a Hallmark of Lysosomal Cell Death Sonja Aits, Marja Jaattela and Jesper Nylandsted 14. Measuring the Phagocytic Activity of Cells Nick Platt and Paul Fineran 15. Detection and Quantification of Microbial Manipulation of Phagosomal Function Maria Podinovskaia, David G. Russell 16. Measuring Relative Lysosomal Volume for Monitoring Lysosomal Storage Diseases Danielle te Vruchte, Kerri L. Wallom and Frances M. Platt 17. Quantifying Storage Material Accumulation in Tissue Sections Jonathan D Cooper, Helen R Brooks, Hemanth R Nelvagal 18. Laboratory Diagnosis of Niemann-Pick disease Type C: The Filipin Staining Test Marie T. Vanier and Philippe Latour

About the Author

Prof. Frances Platt obtained her PhD from the University of Bath, UK, and was a post-doctoral fellow at Washington University Medical School in St. Louis, USA. She was a Lister Institute Senior Research Fellow and a Reader at the University of Oxford. Prof. Platt's main research interests include the biology and pathobiology of glycosphingolipids. Her research led to the development of miglustat for the treatment of glycosphingolipid storage diseases. In 1999, Prof. Platt was awarded the Alan Gordon Memorial Award and the Horst Bickel Award for advances in metabolic disease therapy. She was elected a fellow of the Academy of Medical Sciences in 2011. Dr Platt obtained his PhD from the University of Bath, UK and was a post-doctoral fellow at Washington University in St Louis. Since his return to UK he has worked at University of Oxford in the Sir William Dunn School of Pathology and Nuffield Department of Medicine. He has very recently become a senior research fellow in the Department of Pharmacology. His research interests are in innate immunity, particularly the role of specific pattern recognition receptors in immune and inflammatory responses.

Reviews

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature

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