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Isotope Labeling of Biomolecules - Applications

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This volume of Methods in Enzymology focuses on stable isotope labeling methods and applications for biomolecules

Table of Contents

Nuclear Magnetic Resonance Spectroscopy 1. Application of Natural Isotopic Abundance 1H-13C and 1H-15N Correlated Two- Dimensional NMR for Evaluation of the Structure of Protein Therapeutics Luke W. Arbogast, Robert G. Brinson and John P. Marino 2. A Semi-Automated Assignment Protocol for Methyl Group Side-Chains in Large Proteins Jonggul Kim, Yingjie Wang, Geoffrey Li and Gianluigi Veglia 3. 19F-site Specific Labeled Nucleotides for Nucleic Acid Structural Analysis by NMR Lincoln G. Scott and Mirko Hennig 4. Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as a NMR Assignment Tool and Probe of Homopyrimidine/Homopurine Tract Structural Dynamics Robert G. Brinson, Jennifer T. Miller, Jason D. Kahn, Stuart F. J. Le Grice and John P. Marino Scattering Techniques 5. Biomolecular Deuteration for Neutron Structural Biology and Dynamics Michael Haertlein, Martine Moulin, Juliette M. Devos, Valerie Laux, Orla Dunne, V. Trevor Forsyth 6. Deuterium Labeling Together with Contrast Variation Small-angle Neutron Scattering Suggests How Skp Captures and Releases Unfolded Outer Membrane Proteins Nathan R. Zaccai, Clifford W. Sandlin, James T. Hoopes, Joseph E. Curtis, Patrick J. Fleming, Karen G. Fleming and Susan Krueger 7. Deuteration in Biological Neutron Reflectometry Frank Heinrich 8. Deuterium Labelling Strategies for Creating Contrast in Structure-Function Studies of Model Bacterial Outer Membranes Using Neutron Reflectometry Anton P. Le Brun, Luke A. Clifton, Stephen A. Holt, Peter J. Holden, and Jeremy H. Lakey 9. Essential Strategies for Revealing Nanoscale Protein Dynamics By Neutron Spin Echo Spectroscopy David J. E. Callaway and Zimei Bu Mass Spectrometry 10. Method for the Determination of 15N Incorporation Percentage in Labeled Peptides and Proteins Eric L. Kilpatrick 11. QconCAT: Internal Standard for Protein Quantification Kerry Bauer Scott, Illarion V. Turko and Karen W. Phinney 12. Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark S. Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday and Miral Dizdaroglu Hydrogen-Deuterium Exchange Mass Spectrometry 13. Hydrogen Exchange Mass Spectrometry Leland Mayne 14. Mapping Protein-Ligand Interactions with Proteolytic Fragmentation, Hydrogen/Deuterium Exchange-Mass Spectrometry Elyssia S. Gallagher and Jeffrey W. Hudgens 15. Isotope Labeling of Biomolecules "Structural analysis of viruses by HDX-MS" Miklos Guttman and Kelly K. Lee

About the Author

Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). He is also an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park and affiliated with the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine. Zvi earned a B.Sc. in Agriculture from the Hebrew University in Jerusalem and a M.Sc. in Cell Biology from the Weizmann Institute of Science. After receiving a Ph.D. in Molecular Biology from Cornell University, he was a Helen Hay Whitney Foundation Post-Doctoral Fellow at Johns Hopkins University and Memorial Sloan-Kettering Cancer Center. Upon completion of his postgraduate training, he became a Life Technologies Professor at the Center for Advanced Research in Biotechnology (CARB), University of Maryland Biotechnology Institute (UMBI). Zvi moved to the University of Maryland, College Park in 2010 as a Professor in the Department of Cell Biology and Molecular Genetics. In 2011 he was recruited to NIST to direct the Biomolecular Labeling Laboratory.


Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." - CHEMISTRY IN INDUSTRY "The work most often consulted in the lab." - ENZYMOLOGIA "The Methods in Enzymology series represents the gold-standard." - NEUROSCIENCE

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