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Introduction to BioMEMS
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Table of Contents

How Do We Make Small Things?
Why Bother Making Things Small?
From Art to Chips
Photolithography
Micromachining
Micromolding
Soft Lithography
Hydrogel Devices
Nanofabrication Techniques
Fabrication Based on Self-Assembly: A "Bottom-Up" Approach

Micropatterning of Substrates and Cells
Interaction between Surfaces and Biomolecules
Surface Engineering
Micropatterns of SAMs
Micropatterns of Proteins
Micropatterns of Cells on Nonbiomolecular Templates
Micropatterns of Cells on Biomolecular Templates

Microfluidics
Why Go Small?
Microscale Behavior of Fluids
Fluids in Electrical Fields
Fluids in Acoustic Fields
Fabrication of Microfluidic Channels
Operation of Microfluidic Channels: Practical Concerns
Droplet Microfluidics
Active Flow Control
Micromixers
Combinatorial Mixers

Molecular Biology on a Chip
The Importance of Miniaturizing Molecular Biology
The Importance of Point-of-Care Diagnostics: Where is Cost Really, Really, Really Important?
Sample Preparation: A Bloody Example
The Problem with Microfluidic Sample Separation
Microfluidic Immunoassays
Chips for Genomics and Proteomics
Electrospray Mass Spectrometry
Biochemical Analysis Using Force Sensors

Cell-Based Chips for Biotechnology
Microfluidic Flow Cytometers
Cell Sorting
Cell Trapping
Microfluidic Cell Culture Laboratories
Gene Expression Cellular Microarrays ("Cellomics")
Micro-Bioreactors
Cells on Microelectrodes
Patch Clamp Chips
Cryopreservation
Assisted Reproductive Technologies
Whole Animal Testing

BioMEMS for Cell Biology
An Enabling Technology: The Hurdles
Cell-Substrate Signaling
Cell-Cell Communication
Cell Migration
BioMEMS for Cellular Neurobiology
Developmental Biology on a Chip
Yeast Biology
Plant Cell Biology
Microfluidics for Studying Cellular Dynamics

Tissue Microengineering
Microscaffolding
Micropatterned Cocultures
Stem Cell Engineering
Morphogenesis

Implantable Microdevices
Dental Implants
Implantable Microelectrodes
Delivery of Soluble Signals into the Body
Microtools for Surgery
Insect Research

Appendix Index A Summary and Further Reading appear at the end of each chapter.

About the Author

Albert Folch is an associate professor in the Department of Bioengineering at the University of Washington. Dr. Folch has previously worked as a postdoc researcher at Harvard University's Center for Engineering in Medicine, a postdoc researcher at MIT, and a visiting scientist at the Lawrence Berkeley National Laboratory. He is a recipient of an NSF CAREER Award and is on the advisory board of Lab on a Chip. His research focuses on the interface between cell biology and microfluidics.

Reviews

This book successfully integrates microfabrication and the physics of microfluidics with numerous biological applications. It provides a visual feast of images that lead to a comprehensive understanding of many complex concepts. Applications presented range from biomolecules to cells, tissues, and organisms, and are presented in clear, logical steps. It serves as an outstanding text for advanced undergraduates and graduate students, as well as an excellent review for researchers who are new to the field. --Dr. Fawwaz Habbal, Harvard University An excellent, easy-to-ready textbook for everyone who wants to obtain a fundamental understanding of BioMEMS technology and get inspired about micro/nano bioengineering. --Professor Nikos Chronis, University of Michigan With extensive citations of the current literature, explanations that really put the technology in context, clear enunciation of the motivations for miniaturization, and historical perspective, the author has made the material very accessible and clear ! . I definitely recommend this textbook for bioengineering students. --Professor Karen C. Cheung, University of British Columbia

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