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Human Cell Culture Protocols


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1. Introduction to Cell Culture Christina Philippeos, Robin D. Hughes, Anil Dhawan, and Ragai R. Mitry 2. Isolation and Cultivation of Dermal Stem Cells that Differentiate into Functional Epidermal Melanocytes Ling Li, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn 3. Isolation, Cultivation, and Application of Human Alveolar Epithelial Cells Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr 4. Culture of Parathyroid Cells Peyman Bjoerklund and Per Hellman 5. Functional Analysis of Human Islets of Langerhans Maintained in Culture Shanta J. Persaud, Bo Liu, and Peter M. Jones 6. Conversion of Non-Endocrine Human Pancreatic Cells to Insulin Producing Cells for Treatment of Diabetes Min Zhao and Guocai Huang 7. Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro Maria Jose Gomez-Lechon, Agustin Lahoz, Jose V.Castell, and Maria Teresa Donato 8. Human Hepatocytes: Isolation, Culture, and Quality Procedures Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Buchler, Michael Schoenberg, Joerg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andreas K. Nussler 9. Preclinical Testing of Virotherapeutics for Primary and Secondary Tumors of the Liver Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer 10. Isolation and Functional Studies of Human Fetal Gastric Epithelium in Primary Culture Pierre Chailler, Jean-Francois Beaulieu, and Daniel Menard 11. Isolation, Characterization, and Culture of Normal Human Intestinal Crypt and Villus Cells Jean-Francois Beaulieu and Daniel Menard 12. Primary Culture of Human Renal Proximal Tubule Epithelial Cells and Interstitial Fibroblasts Claire C. Sharpe and Mark E.C. Dockrell 13. Glomerular Epithelial and Mesangial Cell Culture and Characterization Heather M. Wilson and Keith N. Stewart 14. Isolation and Culture of Human Adipocytes and Adipose Tissue Kirstin A. Carswell, Mi-Jeong Lee, and Susan K. Fried 15. Primary Culture of Human Adipocyte Precursor Cells: Expansion and Differentiation Thomas Skurk and Hans Hauner 16. Primary Culture of Ovarian Cells for Research on Cell Interactions in the Hormonal Control of Steroidogenesis Jerzy F. Galas 17. Human Vascular Smooth Muscle Cell Culture Diane Proudfoot and Catherine Shanahan 18. Culture of Human Endothelial Cells from Umbilical Veins Richard C.M. Siow 19. Human Peripheral Blood Mononuclear Cell Culture for Flowcytometric Analysis of Phosphorylated Mitogen-Activated Protein Kinases Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos 20. Human CD4+CD25highCD127low/neg Regulatory T Cells Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma 21. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Darren A. Plumb, and Mary B. Goldring 22. Isolation and Culture of Human Osteoblasts Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, and James A. Gallagher 23. Human Osteoclast Culture and Phenotypic Characterization Ankita Agrawal, James A. Gallagher, and Alison Gartland 24. Effects of Temperature Generated from the Holmium:YAG Laser on Human Osteoblasts in Monolayer Tissue Culture Moustafa I. Hafez, Anne Sandison, Richard R.H. Coombs, Ian D.McCarthy, and Al-Shymaa M. Hafez 25. Laser Microdissection Microscopy: Application to Cell Culture Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia 26. Automated Adherent Human Cell Culture (Mesenchymal Stem Cells) Robert Thomas and Elizabeth Ratcliffe 27. Culturing and Differentiating Human Mesenchymal Stem Cells for Biocompatible Scaffolds in Regenerative Medicine William R. Otto and Catherine E. Sarraf

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