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Flow Cytometry Basics for the Non-Expert


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Table of Contents

1. Flow Cytometry: Definition, History, and Uses in Biotechnology.- 2. Physics of a Flow Cytometer.- 3. The Language of Flow Cytometry and Experimental Setup.- 4. Fluorochrome Choices for Flow Cytometry.- 5. Compensation.- 6. Primary and Seconardy Antibodies and Their Use in Flow Cytometry.- 7. Experimental Considerations with Data Sets as Examples.- 8. Cell Enrichment.- 9. Surface and Intracellular Staining Protocols for Flow Cytometry.- 10. Troubleshooting.

About the Author

Christine Goetz, Ph.D., Jody Bonnevier, Ph.D., and Christopher Hammerbeck, Ph.D.

R&D Systems, Minneapolis, Minnesota

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