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Biomarker Methods in Drug Discovery and Development

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Table of Contents

Table of Content Preface Contributors Chapter 1 Biomarker sample collection and handling in the clinical setting to support early phase drug development Chris B. Russell, Sid Suggs, Kristina M. Robson, Keith Kerkof, Lisa D. Kivman, Kimberly H. Notari, William A. Rees, Natalia Leshinsky, and Scott D. Patterson Chapter 2 Gene Expression-Based Biomarkers of Drug Safety Eric A.G. Blomme, and Scott E. Warder Chapter 3 Profiling Gene Expression Signatures Using Fluorescent Microspheres Suzanne M. Torontali, Jorge M. Naciff, Kenton D. Juhlin, and Jay P. Tiesman Chapter 4 Real-Time PCR Gene Expression Assays in Biomarker Discovery and Validation Yulei Wang, Catalin Barbacioru, David Keys, Pius Brzoska, Caifu Chen, Kelly Li, and Raymond R. Samaha Chapter 5 SAGE Analysis in Identifying Phenotype SNPs Sandro J. de Souza, I-Mei Siu, Janete M. Cerutti, and Gregory J. Riggins Chapter 6 SNP and DNA Methylation Analysis using Pyrosequencing Methods Jinsheng Yu, and Sharon Marsh Chapter 7 The Application of LCM for the Analysis of Specific Cell-Type Gene Expression in a Complex Tissue: the Primate Endometrium William C. Okulicz Chapter 8 The use of Two-Dimensional Gel Electrophoresis for Plasma Biomarker Discovery Brad Jarrold, Alex Varbanov, and Feng Wang Chapter 9 Difference In Gel Electrophoresis (DIGE), a high resolution protein biomarker research tool David S. Gibson, David Bramwell, and Caitriona Scaife Chapter 10 Label-free Mass Spectrometry-based Protein Quantification Technologies in Protein Biomarker Discovery Mu Wang, Jin-Sam You, Kerry G. Bemis, and Dawn P. G. Fitzpatrick Chapter 11 Top-Down Quantitative Proteomic Analysis using a Highly Multiplexed Isobaric Mass Tagging Strategy Bing Xie, Wayne F. Patton, and Craig E. Parman Chapter 12 SELDI Technology for Identification of Protein Biomarkers Prasad Devarajan, and Gary F. Ross Chapter 13 Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High Throughput Screens Rachel M Gonzalez, Susan M Varnum, and Richard C Zangar Chapter 14 LC-MS Metabonomics Methodology in Biomarker Discovery Xin Lu, and Guowang Xu Chapter 15 GC/MS-based metabolomics Sally-Ann Fancy, and Klaus Rumpel Chapter16 NMR-Based Metabolomics for Biomarker Discovery Narasimhamurthy Shanaiah, Shucha Zhang, M. Aruni Desilva, and Daniel Raftery Chapter 17 Unraveling Glycerophospholipidomes by Lipidomics Kim Ekroos Index


From the reviews: "... extremely useful to pharmaceutical scientists who are involved in research all the way from early drug discovery through clinical trials." - Doody's Book Review "The book is ! for the bench scientist and is full of helpful advice on how to achieve high quality data using up-to-date technical approaches in genomics, proteomics and metabonomics. ! I warmly recommend this ! to all practicing toxicologists." (Roy Forster, British Toxicological Society Newsletter, June-August, 2009)

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