Laboratory Experiments in Microbiology http://www.fishpond.co.nz/Books/Laboratory-Experiments-Microbiology-Ted-R-Johnson-Christine-L-Case/9780321560285

By Ted R. Johnson, Christine L. Case

About the Author

Ted R. Johnson is a professor of biology at St. Olaf College, a liberal arts college in Northfield, Minnesota, where he teaches courses in microbiology and immunology. He has taught at St. Olaf for 31 years and previously taught at Mankato State University. He received his master's degree and his Ph.D in microbiology from the University of Illinois in Chicago, Illinois. While at St. Olaf College, he has developed and directed several abroad semester and interim programs. His research focuses on the immune response to virally induced cancer in various animal models. Christine L. Case is a registered microbiologist and a professor of microbiology at Skyline College in San Bruno, California, where she has taught for the past 38 years. She received her Ed. D. in curriculum and instruction from Nova Southeastern University and her M.A. in microbiology from San Francisco State University. She was Director for the Society for Industrial Microbiology (SIM). She received the ASM and California Hayward outstanding educator awards and SACNAS Mentor award. In addition to teaching, Chris contributes regularly to professional literature, develops innovative educational methodologies, and maintains a personal and professional commitment to conservation and the importance of science in society. Chris is also an avid photographer, and many of her photographs appear in this lab manual.

Table of Contents

Introduction I. MICROSCOPY 1. Use and Care of the Microscope 2. Examination of Living Microorganisms II. Handling Bacteria 3. Microbes in the Environment 4. Transfer of Bacteria: Aseptic Techniques III. STAINING METHODS 5. Preparation of Smears and Simple Staining 6. Negative Staining 7. Gram Staining 8. Acid-fast Staining 9. Structural Stains (Endospore, Capsule, Flagella) 10. Morphologic Unknown IV. CULTIVATION OF BACTERIA. 11. Isolation of Bacteria by Dilution Technique 12. Special Media for Isolating Bacteria V. MICROBIAL METABOLISM 13. Carbohydrate Catabolism 14. Fermentation 15. Protein Catabolism, Part 1 16. Protein Catabolism, Part 2 17. Respiration 18. Unknown Identification and Bergey's Manual VI. MICROBIAL GROWTH 19. Oxygen and the Growth of Bacteria 20. Determination of a Bacterial Growth Curve: The Role of Temperature 21. Biofilms VII. CONTROL OF MICROBIAL GROWTH 22. Physical Methods of Control: Heat 23. Physical Methods of Control: Ultraviolet Radiation 24. Chemical Methods of Control: Disinfectants and Antiseptics 25. Chemical Methods of Control: Antimicrobial Drugs 26. Effectiveness of Hand Scrubbing VIII. MICROBIAL GENETICS 27. Regulation of Gene Expression 28. Isolation of Bacterial Mutants 29. Transformation of Bacteria 30. DNA Fingerprinting 31. Genetic Engineering 32. Ames Test for Detecting Possible Chemical Carcinogens IX. THE MICROBIAL WORLD 33. Fungi: Yeasts and Molds 34. Phototrophs: Algae and Cyanobacteria 35. Protozoa 36. Parasitic Helminths X. VIRUSES 37. Isolation and Titration of Bacteriophages 38. Plant Viruses XI. INTERACTION OF MICROBE AND HOST 39. Epidemiology 40. Koch's Postulate XII. IMMUNOLOGY 41. Nonspecific Resistance 42. Blood Group Determination: Slide Agglutination 43. Agglutination Reactions: Microtiter Agglutination 44. ELISA Technique XIII. MICROORGANISMS AND DISEASE 45. Bacteria of the Skin 46. Bacteria of the Respiratory Tract 47. Bacteria of the Mouth 48. Bacteria of the Gastrointestinal Tract 49. Bacteria of the Genitourinary Tract 50. Rapid Identification Methods 51. Identification of an Unknown from a Clinical Sample XIV. MICROBIOLOGY AND THE ENVIRONMENT 52. Microbes in Water: Multiple-Tube Technique 53. Microbes in Water: Membrane Filter Technique 54. Microbes in Food: Contamination 55. Microbes Used in the Production of Foods 56. Microbes in Soil: The Nitrogen and Sulfur Cycles 57. Microbes in Soil: Bioremediaton APPENDICES A. Pipetting B. Dilution Techniques and Calculations C. Use of the Spectrophotometer D. Graphing E. Use of the Dissecting Membrane F. Use of the Membrane Filter G. Electrophoresis H. Keys to Bacteria Index